熒光/化學(xué)光成像儀


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【Cool Imager?】- 高靈敏生物發(fā)光與熒光數(shù)字成像儀 (IMGR002, IMGR003)

一、產(chǎn)品背景常規(guī)的生物圖像與生物信號用肉眼觀察靈敏度低或根本無法進行肉眼觀察。常常需要采用酶顯色或放射性同位素放大后進行觀察。近年興起的Chemiluminescence(化學(xué)發(fā)光)因其極高靈敏度與優(yōu)越的線性關(guān)系得到普遍應(yīng)用。用光學(xué)照相機照像或膠片顯影法顯示結(jié)果則需要暗室以及膠片,照相紙,沖洗液等耗材。圖像的定量分析與后期處理也較困難。相比之下,通過數(shù)字相機的數(shù)字成像技術(shù)則有許多優(yōu)點。國內(nèi)市場上出售的電泳圖像成像系統(tǒng)因靈敏度低而不能用于顯示化學(xué)自發(fā)光反應(yīng) (Chemiluminescence)以及熒光示蹤。結(jié)合現(xiàn)代生物學(xué),光學(xué),半導(dǎo)體及計算機技術(shù),我公司開發(fā)了高靈敏度的數(shù)字成像系統(tǒng)(Cool Imager)。 Cool Imager為多用途數(shù)字成像及圖像分析工作站,主要用于酶催化的化 學(xué)自發(fā)光反應(yīng)與熒光圖譜。由于對攝像系統(tǒng)采用了深低溫冷卻(-45oC)技術(shù),靈敏度較傳統(tǒng)的酶底物顯色法提高約2000倍。

完全成像系統(tǒng)包括所有硬件、并已經(jīng)安裝系統(tǒng)與分析軟件,開箱后即可使用。高度集成的系統(tǒng)已經(jīng)包括微機,不需要另配計算機。系統(tǒng)已經(jīng)優(yōu)化。系統(tǒng)啟動與關(guān)閉迅速。采像單元可每分鐘采積并累加近2000幅圖。最高累加圖幅達(dá)200,000幅圖,采像時間可達(dá)25分鐘。一般取圖5,000幅左右即可在計算機屏幕清晰顯示蛋白印跡圖或分子雜交圖。由于采圖數(shù)是任意可變的,Cool Imager可擴展用于攝取DNA/RNA凝膠圖,考馬氏亮蘭及銀染的蛋白凝膠圖,以及蛋白熒光圖譜。

本工作臺可連接顯微攝像頭攝取彩色顯微鏡圖像。
  

二、技術(shù)指標(biāo)

系統(tǒng)冷卻達(dá)-45oC,可以使微弱的發(fā)光成像。曝光25分鐘、累集10,000幅圖沒有明顯雜色背景。
  

三、技術(shù)參數(shù)(CoolImager II)

 

 

 

 

 

 

 

 

 

 

MAIN UNIT

Complete System

Cool Imager™ is an all-in-one unit and comes with all the hardware including the monitor, keyboard, mouse, pre-installed operating system and analytical software. After you receive the units, plug in and use; that is it. No custom hardware and software installation are required.

High Integration

The unit is integrated with the computer-board with an embedded CPU, the control board and operating panel, optical devises, a frame grabber, lights, filters and a mini darkroom chamber. This specific design increases efficiency and stability of the workstation.

Low-noise

Technology

Low-noise technology is applied to all the electronic components in the unit to prevent the interruption of electro-noise and reduce vibration, which makes the design of highly integrated system possible and enhances the stability of the unit.

Sensitivity

Detects as little as ~0.5 fg (10-17g) of HRP with Lightgen™ substrates and completely eliminates the use of X-ray film for chemiluminescent imaging.

Imaging Capacity

Cool imager can integrate ~30 frames per second and collect ~50,000 frames in ~30min, and its sensitivity is better than autoradiography using radioisotope p32 or S35.

Darkroom

The mini darkroom is light-proofed by a design with multi-staged, magnetic sealing strips, which permit the door to open and close easily.

Self-contained

Unit

There is no external camera or lens outside of the unit. All electronic and optical components are enclosed inside the unit, which provides a moisture-proof, dust-proof environment and extend the service life of the electronic and optical components.

Power

Operation

Workstation uses a flat control panel to turn on and off the operation program and also controls image capturing, light source, power lens and camera functions.

 

 

 

SOFT-Ware

Operation

Pre-installed Windows® (Microsoft) or Linux®  (Redhat) operation program

Imaging & Analyzing Software

With the software, ViaCapture, capturing an image is achieved simply by entering the number of integration. 1D gel, 2D dot-blot, bacterial colony, Western blots, DNA/RNA hybridization, EMSA and microplates can be imaged and saved by Cool Imager™. The captured images can be opened and edited with ViaAnalysis software. ViaAnalysis can invert, correct slanted images, adjust image contrast and brightness, perform image quantitation and more. All images can be copied, pasted or printed.

Other programs

Office, Image editor, PDF, Zip and Antivirus programs are pre-installed.

C C D

The ultra-cooled, C-Mount camera with 2-stage Peltier cooling mechanism, cools temperature down to -45oC and reduces the background noise down to 17 e- rms. The cooled camera has an 8 bit, ½ inch CCD installed with a pixel size of 6.4um X 6.4um. The resolution can reach up to 1642H X 1024V.

LENS

F=1:1.0, 8~48mm optical lens with power zoom, power focus and iris.

LIGHTS

A set of white reflective lights and 3 sets of colored reflective lights (Cold Light) are included. Transmitting blue, ultra-violet or white light boxes are sold separately at an additional cost.

FILTERS

A tunable filter wheel which can hold 4 sets of filters. A brown filter is included for imaging DNA/RNA gels stained with Ethidium bromide or SYBR Green. Other filters are options sold separately at an additional cost.

注; 攝像機的冷卻溫度與儀器靈敏度有關(guān)。溫度越低,雜色越少,靈敏度越高,價格高。 VersaDoc未配置變焦鏡頭,沒有圖像放大功能,不能用于基因芯片圖。 CoolImager配專門技術(shù)用于我公司推出的EMSA系列成套試劑。其他公司的儀器不能顯示結(jié)果。Cool Imager本身具有所有控制單元的軟,硬件。只需外接顯示器即可使用。

 

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